C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/073 (2010.01) C12N 5/071 (2010.01) C12N 5/0735 (2010.01) A61K 35/407 (2006.01) A61P 1/16 (2006.01) C12Q 1/02 (2006.01)
Patent
CA 2580798
The present invention relates to methods for obtaining endodermal progenitor cells and further differentiating these to hepatocyte-like cells. Knowledge about the cell composition prior to the initiation of terminal differentiation is used to select one of two different protocols and one of two type of intermediate progenitors depending on the purpose for which the resulting hepatocyte-like cells are needed. Protocol A of the present invention relates to differentiation of extraembryonic-resembling endodermal progenitor cells to hepatocyte-like cells and may be selected when yield and purity of the obtained hepatocyte-like cells is important. Protocol B of the present invention relates to differentiation of mesendodermal-resembling progenitor cells to hepatocyte-like cells and may be selected when quality of the obtained hepatocyte-like cells is important.
La présente invention concerne l'obtention de cellules progénitrices endodermiques puis, par différentiation, de cellules ressemblant aux hépatocytes. Connaissant la composition de la cellule avant le commencement de la différentiation finale, on peut sélectionner l'un de deux protocoles différents et l'un de deux types de cellules progénitrices intermédiaires en fonction de la raison pour laquelle on a besoin des cellules ressemblant aux hépatocytes résultantes. Le protocole A de la présente invention se rapporte à l'obtention de cellules ressemblant aux hépatocytes par différentiation de cellules progénitrices endodermiques ressemblant aux cellules de la membrane extra-embryonnaire. On choisira ce protocole lorsqu'on met l'accent sur le rendement et le pureté des cellules ressemblant aux hépatocytes obtenues. Le protocole B de la présente invention se rapporte à l'obtention de cellules ressemblant aux hépatocytes par différentiation de cellules progénitrices ressemblant aux cellules du mésendoderme. On choisira ce protocole lorsqu'on met l'accent sur la qualité des cellules ressemblant aux hépatocytes obtenues.
Akesson Karolina
Heins Nico
Rehnstrom Marie
Semb Henrik
Cellartis Ab
Fetherstonhaugh & Co.
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