Methods for the identification of inhibitors of...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/60 (2006.01) C07K 14/37 (2006.01) C12N 9/02 (2006.01) C12N 9/10 (2006.01) C12N 9/88 (2006.01) C12N 15/31 (2006.01) C12N 15/53 (2006.01) C12Q 1/02 (2006.01) C12Q 1/18 (2006.01) C12Q 1/26 (2006.01) C12Q 1/527 (2006.01) G01N 33/573 (2006.01)

Patent

CA 2469772

The present inventors have discovered that .alpha.-Aminoadipate Reductase and Homocitrate Synthase are each essential for fungal pathogenicity. Specifically, the inhibition of .alpha.-Aminoadipate Reductase or Homocitrate Synthase gene expression in fungi results in no signs of successful infection or lesions. Thus, .alpha.-Aminoadipate Reductase or Homocitrate Synthase can be used as a target for the identification of antibiotics, preferably antifungals. Accordingly, the present invention provides methods for the identification of compounds that inhibit .alpha.-Aminoadipate Reductase or Homocitrate Synthase expression or activity. The methods of the invention are useful for the identification of antibiotics, preferably antifungals.

Selon l'invention, l'.alpha.-aminoadipate réductase et l'homocitrate synthase sont chacune essentielle concernant la pathogénicité fongique. Plus spécifiquement, l'inhibition de l'expression génique de l'.alpha.-aminoadipate réductase ou de l'homocitrate synthase chez les champignons résulte en l'absence de signe d'infection ou de lésions. Ainsi l'.alpha.-aminoadipate réductase ou l'homocitrate synthase peuvent être utilisées comme cibles dans l'identification d'antibiotiques, de préférence antifongiques. En conséquence, l'invention concerne des procédés d'identification de composés inhibiteurs de l'expression ou de l'activité de l'.alpha.-aminoadipate réductase ou de l'homocitrate synthase. Ces procédés sont utiles pour l'identification d'antibiotiques, de préférence antifongiques.

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