Methods for the isolation and proliferation of...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/08 (2006.01) C12N 5/06 (2006.01) C12Q 1/02 (2006.01) C12Q 1/24 (2006.01)

Patent

CA 2258499

Methods have been developed for isolating and proliferating insulin-secreting mammalian cells. Invention methods involve isolating insulin-secreting cells and preparing a primary culture in which insulin is secreted under the control of one or more insulin promoter transcription factors, e.g., NKX 6.1, HNF3- beta, STF-1, Beta 2, and the like. Such proliferated insulin-secreting cells are distinguished from all other cells in that they are proliferated and in that they have measurable intracellular levels of one or more transcription factors associated with insulin production, as can readily be determined by suitable assays.

L'invention concerne des procédés destinés à l'isolation et à la prolifération de cellules mammaliennes sécrétant de l'insuline. Les procédés de l'invention comprennent l'isolation de cellules sécrétant de l'insuline et la préparation d'une culture primaire dans laquelle de l'insuline est sécrétée, sous la régulation d'un ou de plusieurs facteurs de transcription promoteurs de l'insuline, comme par exemple, NKX 6.1, HNF3-beta, STF-1, Beta 2, et autres facteurs similaires. Ces cellules de prolifération sécrétant de l'insuline se distinguent de toutes les autres cellules en ce qu'elles sont issues d'une prolifération et qu'elles ont des niveaux intracellulaires mesurables d'un ou de plusieurs facteurs de transcription associés à la production d'insuline, ainsi que peuvent facilement le démontrer des essais appropriés.

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