C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 14/81 (2006.01)
Patent
CA 2294613
A rapid and simple method of isolating heat stable proteinase inhibitor proteins from plant tissues such as potato tubers is disclosed. The method comprises three steps. Proteins from potato tubers are extracted in an aqueous/alcohol extraction medium to form an alcohol extract. The alcohol extract is heated to a first temperature then cooled to a second temperature to form an insoluble precipitate phase containing debris and a soluble phase that contains the heat stable proteinase inhibitor proteins. The heat stable proteinase inhibitor proteins are precipitated from the soluble phase by dialysis against a suitable dialysis medium. The precipitated proteins may include a single inhibitor protein, or a mixture thereof.
L'invention concerne un procédé simple et rapide permettant d'isoler des protéines thermostables inhibitrices de protéinase tirées de tissus végétaux tels que les tubercules de pomme de terre. Le procédé comporte trois étapes. On extrait les protéines des tubercules de pomme de terre dans un milieu d'extraction eau/alcool pour former un extrait d'alcool. On chauffe cet extrait d'alcool à une première température, puis on le refroidit à une seconde température pour former une phase de précipité insoluble renfermant des débris, et une phase soluble renfermant les protéines thermostables inhibitrices de protéinase. On précipite ces protéines thermostables inhibitrices de protéinase à partir de la phase soluble par dialyse dans un milieu de dialyse approprié. Les protéines précipitées peuvent contenir une seule protéine inhibitrice, ou un mélange de ces protéines.
Pearce Gregory L.
Ryan Clarence A.
Gowling Lafleur Henderson Llp
Washington State University Research Foundation
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