Methods for the preparation of dna microarrays with linear...

Details Methods for the preparation of dna microarrays with linear... Methods for the preparation of dna microarrays with linear...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2699506

A method for the realisation of DNA microarrays with linear high density probes, comprising a phase of replication of template DNA strands of a template microarray and a subsequent phase of stamping of the molecules obtained by replication on a substrate via the SuNS technique; the template strands (21, 38, 44) of said template microarray are either composed of or derived from a rolling circle (21; 38; 43).

L'invention concerne un procédé de préparation de microréseaux d'ADN à sondes haute densité linéaire. Ce procédé comprend : une phase de réplication de brins de matrice d'ADN d'un microréseau de matrice; et une phase ultérieure de pressage des molécules obtenues par réplication sur un substrat, par la mise en uvre de la technique SuNS. Les brins de matrice (21, 38, 44) du microréseau de matrice sont soit constitués par un cercle roulant soit issus dudit cercle (21; 38; 43).

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