Methods for the preparation of polynucleotide librairies and...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/00 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12N 15/10 (2006.01) C12N 15/63 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2442096

Methods of directed fragmentation of polynucleotides combined with fragment interchange and ligation are provided for the preparation of polynucleotide libraries as shown in figure 1. Fragmentation can be facilitated by at least one oligonucleotide adapter capable of directing polynucleotide cleavage at homologous sites among a set of parent polynucleotides. Libraries generated by the above methods can be screened for polynucleotides with desired characteristics or properties.

La présente invention concerne des procédés permettant une fragmentation dirigée de polynucléotides se combinant à un échange des fragments et à leur ligation, en vue de l'élaboration d'échantillothèques de polynucléotides tel que le représente la figure 1. La fragmentation peut être facilitée par au moins un oligonucléotide d'adaptation capable de diriger le clivage des nucléotides en des sites homologues pris dans un jeu de polynucléotides parents. Les échantillothèques obtenues selon ces procédés se prêtent à la recherche systématique de polynucléotides aux caractéristiques ou propriétés attendues.

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