C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/86 (2006.01) A61K 31/70 (2006.01) A61K 48/00 (2006.01) C07K 16/10 (2006.01) C07K 16/18 (2006.01) C12N 5/06 (2006.01) C12N 5/10 (2006.01) C12N 7/01 (2006.01) C12N 15/867 (2006.01)
Patent
CA 2195309
Methods and compositions are provided for facilitating gene therapy procedures involving the transduction of target cells with retroviral vector particles in the presence of complement containing body fluids. The reduction of levels of galactose alpha (1, 3) galactosyl epitopes on the retroviral vector particles and/or the blockade of antibody binding to such epitopes have been found to render the particles less sensitive to inactivation by complement mediated mechanisms, and to thus allow transduction in the presence of complement containing body fluids. Means are provided for obtaining such reductions.
On décrit des procédés et compositions destinés à faciliter des procédures de thérapie génique et impliquant la transduction de cellules cibles avec des particules de vecteurs retroviraux en présence de fluides corporels contenant des compléments. La réduction des niveaux d'épitopes de galactose alpha (1, 3) galactosyle présents sur ces particules de vecteurs rétroviraux et/ou le blocage de la liaison d'anticorps à ces épitopes a permis de constater une moindre sensibilité de ces particules à une inactivation par des mécanismes de médiation par ces compléments, ce qui permet une transduction en présence de fluides corporels, contenant de tels compléments. On décrit des procédures qui donnent de telles réductions.
Fodor William L.
Rollins Scott A.
Rother Russell P.
Springhorn Jeremy P.
Squinto Stephen P.
Alexion Pharmaceuticals Inc.
Mcfadden Fincham
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