Methods for the production of apolipoproteins in transgenic...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/12 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) A61K 38/17 (2006.01) A61P 3/06 (2006.01) C07K 14/775 (2006.01) C12N 15/82 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2544239

Methods for the production of an apolipoprotein in plants are described. In one embodiment, the present invention provides a method for the expression of apolipoprotein in plants comprising : (a) providing a chimeric nucleic acid construct comprising in the 5~ to 3~ direction of transcription as operably linked components: (i) a nucleic acid sequence capable of controlling expression in plant cells; and (ii) a nucleic acid sequence encoding an apolipoprotein polypeptide; (b) introducing the chimeric nucleic acid construct into a plant cell; and growing the plant cell into a mature plant capable of setting seed wherein the seed expresses apolipoprotein.

L'invention concerne des procédés pour la production d'une apolipoprotéine dans des plantes. Dans un mode de réalisation, l'invention concerne un procédé pour l'expression de l'apolipoprotéine dans des plantes, consistant : (a) à utiliser une construction chimère d'acides nucléiques comprenant, dans la direction 5' à 3' de transcription, comme composants reliés fonctionnellement (i) une séquence d'acides nucléiques capable de contrôler l'expression dans des cellules végétales, et (ii) une séquence d'acides nucléiques codant pour un polypeptide d'apolipoprotéine, (b) à introduire la construction d'acides nucléiques chimériques dans une cellule végétale et (c) à amener la plante à un état de maturité où elle peut produire des semences qui expriment l'apolipoprotéine.

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