Methods for the production of hybrid seed

Details Methods for the production of hybrid seed Methods for the production of hybrid seed

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) A01H 1/00 (2006.01) A01H 1/02 (2006.01) A01H 5/00 (2006.01) C07K 14/415 (2006.01) C12N 5/10 (2006.01)

Patent

CA 2106718

The present invention provides a dual method for producing male-sterile plants. Two genetically transformed plants, parents 1 and 2 are crossed to obtain male- sterile offspring. Parent 1 is transformed with an expression cassette comprising a nucleotide sequence encoding an anther-specific promoter which is operably linked to a nucleotide sequence encoding a transactivator. Parent 2 is transformed with an expression cassette comprising a target nucleotide sequence, which is capable of being activated by the transactivator, operably linked to a nucleotide sequence which encodes RNA or a polypeptide which will disrupt the formation of viable pollen. Therefore, crossing parent 1 with parent 2 results in male-sterile offspring. The male-sterile plants are useful for producing hybrid seed. The invention also provides compositions and methods for high level expression of a coding region of interest in a plant.

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