Methods for the production of insulin in plants

Details Methods for the production of insulin in plants Methods for the production of insulin in plants

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) A01H 5/00 (2006.01) C07K 14/62 (2006.01)

Patent

CA 2528741

Methods for the production of insulin in plants are described. In one embodiment, the present invention provides a method for the expression of insulin in plants comprising: (a) providing a chimeric nucleic acid construct comprising in the 5~ supl; to 3~ direction of transcription as operably linked components: (i) a nucleic acid sequence capable of controlling expression in plant seed cells; and (ii) a nucleic acid sequence encoding an insulin polypeptide; (b) introducing the chimeric nucleic acid construct into a plant cell; and (c) growing the plant cell into a mature plant capable of setting seed wherein the seed expresses insulin.

Cette invention concerne des méthodes de production d'insuline dans les plantes. Dans un mode de réalisation, l'invention concerne un procédé d'expression de l'insuline dans les plantes, consistant: (a) à utiliser une construction chimère d'acides nucléiques comprenant dans la direction 5' à 3' de transcription, en tant que composants reliés fonctionnement (i) une séquence d'acides nucléiques capable de contrôler l'expression dans des cellules de semences végétales, et (ii) une séquence d'acides nucléiques codant pour un polypeptide d'insuline; (b) à introduire la construction d'acides nucléiques chimériques dans une cellule végétale; et (c) à amener la plante à un état de maturité où elle peut produire des semences qui expriment l'insuline.

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