A - Human Necessities – 01 – H
Patent
A - Human Necessities
01
H
A01H 5/00 (2006.01) A01H 5/10 (2006.01) C12N 15/82 (2006.01) C12N 15/84 (2006.01)
Patent
CA 2230216
Disclosed are processes for producing stably transformed fertile wheat and a system of transforming wheat via Agrobacterium. This invention provides methods for transforming a variety of explants, such as freshly isolated or pre-cultured immature embryos, embryogenic callus and suspension cells. Also disclosed are methods for recovering transgenic plants after transformation within a short period of time, if the explants are regenerable at the time of transformation. Thus, the frequency of somaclonal variation associated with prolonged in vitro culture period is significantly reduced. The transformation frequency using this system is comparable to or better than published methods using other systems, such as microprojectile bombardment.
La présente invention concerne des procédés permettant de produire du blé fertile transformé stable et un système permettant de transformer le blé au moyen de l'Agrobacterium. On décrit des procédés permettant de transformer divers explants, tels que des embryons immatures pré-cultivés et fraîchement isolés, des cals embryonnaires et des cellules en suspension. On décrit également des procédés permettant de regénérer des plantes transgéniques peu de temps après leur transformation, lorsque les explants sont regénérables au moment de la transformation. On réduit de la sorte de manière significative la fréquence des variations somaclonales associées à une période de culture in vitro prolongée. A l'aide de ce système, on obtient une fréquence de transformation comparable ou meilleure que les procédés publiés faisant appel à d'autres systèmes, tels que le bombardement avec des microprojectiles.
Cheng Ming
Fry Joyce E.
Wan Yuechun
Deeth Williams Wall Llp
Monsanto Company
Monsanto Technology Llc
LandOfFree
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