Methods for the production of tcr gamma delta t cells

Details Methods for the production of tcr gamma delta t cells Methods for the production of tcr gamma delta t cells

C - Chemistry, Metallurgy

12

N

C12N 5/0783 (2010.01) A61P 37/02 (2006.01) A61K 35/12 (2006.01)

Patent

CA 2349629

The method for obtaining and expanding TcR.gamma..delta.+ T cells in culture is described. The method involves: 1) culturing cells from a sample containing TcR.gamma..delta.+ T cells or precursors thereof in a first culture medium comprising a T cell mitogen and at least two cytokines and 2) culturing the cells obtained in step 1) in a second culture medium comprising at least two cytokines. Preferably, the method comprises 1) culturing the cells in a first culture medium comprising (a) a T cell mitogen, (b) interleukin-2 and (c) interleukin-4; and 2) culturing the cells obtained in step 1) in a second culture medium comprising (i) interleukin-2 and (ii) interleukin-4 to obtain TcR.gamma..delta.+ T cells. The TcR.gamma..delta.+ T cells obtained by the method can be used in a variety of experimental, therapeutic and commercial applications.

L'invention concerne une méthode permettant d'obtenir et de développer des lymphocytes T TcR gamma delta <+> de culture. Ladite méthode consiste: 1) à cultiver les cellules à partir d'un échantillon renfermant des lymphocytes T TcR gamma delta <+>, ou leurs précurseurs, dans un premier milieu de culture contenant un mitogène des lymphocytes T et au moins deux cytokines et 2) à cultiver les cellules obtenues lors de l'étape 1) dans un second milieu de culture contenant au moins deux cytokines. De préférence, la méthode consiste 1) à cultiver les cellules dans un premier milieu de culture renfermant (a) un mitogène des lymphocytes T, (b) une interleukine-2 et (c) une interleukine-4; et 2) à cultiver les cellules obtenues à l'étape 1) dans un second milieu de culture renfermant (i) une interleukine-2 et (ii) une interleukine-4, de manière à obtenir des lymphocytes T TcR gamma delta <+>. Les lymphocytes T TcR gamma delta <+> obtenus par cette méthode peuvent être utilisés dans diverses applications expérimentales, thérapeutiques et commerciales.

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