C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 14/00 (2006.01) C07K 1/14 (2006.01) C07K 14/245 (2006.01) C07K 14/35 (2006.01) C07K 14/435 (2006.01)
Patent
CA 2398605
Methods are described for the purification and spinning of recombinant and non- recombinant proteins. Specifically, the lysis of bacteria and purification of silk proteins occur in a single solution of organic acid. Bacterial proteins are hydrolyzed while the silk protein remains intact. Silk proteins remain soluble as they are concentrated into an aqueous-based mixture for fiber spinning.
L'invention concerne des procédés pour la purification et le filage de protéines recombinantes et non-recombinantes. Plus spécifiquement, la lyse de bactéries et la purification de protéines de la soie s'effectuent dans une seule solution d'acide organique. Les protéines bactériennes sont hydrolysées, alors que la protéine de la soie demeure intacte. Les protéines de la soie demeurent solubles lorsqu'elles sont concentrées en un mélange de base aqueux pour le filage des fibres.
Arcidiacono Steven
Butler Michelle M.
Mello Charlene M.
Arcidiacono Steven
Butler Michelle M.
Mello Charlene M.
Moffat & Co.
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