Methods for the quantitation of oxidized glutathione

Details Methods for the quantitation of oxidized glutathione Methods for the quantitation of oxidized glutathione

G - Physics

01

N

G01N 33/50 (2006.01) G01N 33/52 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2391244

The present invention is directed to a method for determining the level of oxidized glutathione, or the ratio of reduced glutathione to oxidized glutathione, in a biological sample utilizing the thiol scavenging reagent 1- methyl-2-vinylpyridinium at a level which rapidly scavenges reduced glutathione but does not interfere with the measurement of oxidized glutathione.

La présente invention concerne un procédé permettant de déterminer la teneur en glutathione oxydé ou le taux du glutathione oxydé par rapport au glutathione réduit dans un échantillon biologique au moyen d'un réactif 1-méthyl-2-vinylpyridinium capteur de thiols, qui est présent dans des proportions lui permettant de capter rapidement le glutathione réduit sans pour autant interférer avec la mesure du glutathione oxydé.

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