Methods for the recombinant production of antifusogenic...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/62 (2006.01) A61K 38/16 (2006.01) C07K 14/08 (2006.01) C07K 14/155 (2006.01) C07K 14/16 (2006.01) C07K 19/00 (2006.01) C12N 15/09 (2006.01) C12N 15/33 (2006.01) C12N 15/48 (2006.01) C12N 15/49 (2006.01) C12N 15/63 (2006.01) C12P 21/02 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2443365

A process for the recombinant production of an antifusogenic peptide by expression of a nucleic acid encoding said antifusogenic peptide as repeat peptide in a microbial host cell, isolation of inclusion bodies containing said repeat peptide, solubilization of the inclusion bodies, and isolation of the antifusogenic peptide after cleavage, characterized by washing the inclusion bodies with a denaturing agent at a pH value of or below pH 6.5, solubilizing said inclusion bodies containing the repeat peptide at a pH value of at least pH 9 and cleaving said repeat peptide to obtain said antifusogenic peptide.

Un procédé permettant la production par recombinaison d'un peptide antifusogène qui consiste à exprimer un acide nucléique codant ledit peptide antifusogène en tant que peptide de répétition dans une cellule hôte microbienne, à isoler les corps d'inclusion contenant ledit peptide de répétition, à solubiliser des corps d'inclusion et à isoler le peptide antifusogène après clivage, caractérisé par le levage des corps d'inclusion avec un agent dénaturant à une valeur de pH égale ou inférieure à 6,5, la solubilisation des corps d'inclusion contenant le peptide de répétition à une valeur de pH d'au moins 9 et le clivage dudit peptide de répétition pour obtenir ledit peptide antifusogène.

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