Methods for transfecting natural killer cells

Details Methods for transfecting natural killer cells Methods for transfecting natural killer cells

C - Chemistry, Metallurgy

12

N

C12N 15/86 (2006.01) C12N 15/85 (2006.01) C12N 15/87 (2006.01)

Patent

CA 2560751

Methods for stably transfecting mammalian natural killer cells comprising: transfecting a packaging cell line with a retroviral expression vector; culturing the transfected packaging cell line in a cell culture medium; and culturing the mammalian natural killer cells with the cell culture medium. Natural killer cells transfected according to the disclosed methods are also provided.

L'invention concerne des méthodes qui permettent de transfecter de manière stable des cellules K naturelles mammaliennes. Ces méthode consistent : à transfecter une lignée cellulaire d'enrobage à l'aide d'un vecteur d'expression rétroviral ; à cultiver la lignée cellulaire d'enrobage ainsi transfectée dans un milieu de culture cellulaire ; et à cultiver les cellules K naturelles mammaliennes au moyen de ce milieu de culture cellulaire. L'invention concerne également les cellules K naturelles transfectées conformément aux méthodes selon l'invention.

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