Methods for transferring the capability to produce a natural...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/10 (2006.01) C12N 1/21 (2006.01) C12N 15/52 (2006.01) C12N 15/70 (2006.01) C12N 15/76 (2006.01) C12P 17/18 (2006.01) C12P 19/62 (2006.01)

Patent

CA 2330543

The present invention provides a system for producing and modifying natural products produced by a large group of bacteria for the purpose of drug discovery, development and production. The method of the invention transfers the ability to produce a secondary metabolite from an actinomycete that is the original producer of the natural product, to a different production host that has desirable characteristics. The system involves the construction of a segment of the chromosome of the original producer in an artificial chromosome that can be stably maintained in a suitable production host. The present invention relates to recombinant DNA vectors useful for shuttling the genetic information necessary to synthesize a given natural product between a donor organism and a production host. The methods of the invention are useful in improving the yield, the purification process and for structural modification of a natural product.

L'invention concerne un système de production et de modification de produits naturels obtenus à partir d'un groupe important de bactéries, dans un but de découverte, développement et production de médicaments. Le procédé de l'invention consiste à transférer la capacité de production d'un métabolite secondaire, à partir d'un actinomycète constituant le producteur original du produit naturel, à un hôte de production différent et possédant les caractéristiques voulues. Ce système implique la construction d'un segment du chromosome du producteur original, dans un chromosome artificiel qui peut être maintenu de manière stable dans un hôte de production approprié. L'invention concerne encore des vecteurs d'ADN de recombinaison, utiles en tant que navettes des informations génétiques nécessaires à la synthèse d'un produit naturel donné, entre un organisme donneur et un hôte de production. Ces procédés sont utiles pour améliorer le rendement, le procédé de purification et la modification structurelle d'un produit naturel.

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