C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/79 (2006.01) A01H 13/00 (2006.01) C12N 1/13 (2006.01) C12N 5/10 (2006.01) C12P 7/02 (2006.01) C12N 15/82 (2006.01)
Patent
CA 2682950
Compositions and methods for genetically modifying algae or other photosynthetic eukaryotes to express an alcohol producing enzyme, such as pyruvate decarboxylase (PDC) and alcohol dehydrogenase (ADH) or any other enzymes that also synthesizes methanol, ethanol or butanol are provided. These enzymes are engineered to contain chloroplast targeting sequences which efficiently target xenotypic proteins to the chloroplast. Algae can be stably transformed with the compositions comprising chloroplast targeting constructs containing alcohol producing enzymes whereby the alcohol is produced in the chloroplast of the organism.
Cette invention se rapporte à des compositions et à des procédés permettant de modifier génétiquement des algues ou d'autres eucaryotes photosynthétiques de manière à exprimer une enzyme productrice d'alcool, comme la pyruvate décarboxylase (PDC) et l'alcool déshydrogénase (ADH) ou toute autre enzyme qui synthétise aussi le méthanol, l'éthanol ou le butanol. Ces enzymes sont fabriquées de manière à contenir des séquences ciblant les chloroplastes qui ciblent efficacement les protéines xénotypiques sur le chloroplaste. Les algues peuvent être transformées avec stabilité avec les compositions comprenant des constructions ciblant les chloroplastes contenant des enzymes productrices d'alcool, l'alcool étant produit dans le chloroplaste de l'organisme.
Shapiro Cohen
Tabletop Energy
LandOfFree
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