C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01) G01N 33/68 (2006.01)
Patent
CA 2346965
Identification of proteins correlating to a particular phenotype by screening for intracellular proteins interacting with two different sets of "pertubagens", i.e. proteins altering a cellular phenotype in a transdominant way. Protein interactions are preferably detected by two-hybrid assay. The double two-hybrid screening of the invention eliminates or reduces false positives.
La présente invention concerne une méthode d'identification de protéines se rapportant à un phénotype particulier. Cette méthode consiste à rechercher systématiquement des protéines intracellulaires qui interagissent avec deux ensembles différents de gènes <= perturbants >=, c'est à dire de protéines qui modifient un phénotype cellulaire de façon transdominante. Pour la détection des interactions protéiques, on procède de préférence à des analyses à deux hybrides. Ce double criblage à deux hybrides selon l'invention permet d'éliminer ou de réduire les faux positifs
Arcaris Inc.
Deltagen Proteomics Inc.
Smart & Biggar
LandOfFree
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