Methods of amplifying and sequencing nucleic acids

Details Methods of amplifying and sequencing nucleic acids Methods of amplifying and sequencing nucleic acids

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12P 19/34 (2006.01) C40B 50/06 (2006.01) C40B 50/14 (2006.01) C40B 40/06 (2006.01)

Patent

CA 2727850

A method for sequencing nucleic acids. The method includes fragmenting large template nucleic acid molecules to generate a plurality of fragmented nucleic acids; attaching one strand of a plurality of the fragmented nucleic acids individually to beads to generate single stranded nucleic acids attached individually to beads; delivering a population of the single stranded fragmented nucleic acids attached individually to beads to an array of at least 10,000 reaction chambers on a planar surface, wherein a plurality of the reaction chambers comprise no more than one bead with one single stranded fragmented nucleic acid; amplifying the single stranded nucleic acids in the reaction chambers; and, performing a sequencing reaction simultaneously on a plurality of the reaction chambers.

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