Methods of amplifying sequencing nucleic acids

Details Methods of amplifying sequencing nucleic acids Methods of amplifying sequencing nucleic acids

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12M 1/40 (2006.01) C12P 19/34 (2006.01) C40B 30/00 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2616762

An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.

L'invention concerne un appareil et une méthode permettant d'effectuer un séquençage d'ADN rapide, de type séquençage génomique. La méthode de l'invention comprend les étapes consistant : à préparer un échantillon d'ADN en vue d'un séquençage génomique ; à amplifier l'ADN préparé de manière représentative ; et à effectuer plusieurs réactions de séquençage sur l'ADN amplifié avec une seule étape d'hybridation d'amorce.

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