Methods of applying physical stimuli to cells

Details Methods of applying physical stimuli to cells Methods of applying physical stimuli to cells

C - Chemistry, Metallurgy

12

M

C12M 1/42 (2006.01) C12M 3/00 (2006.01) C12N 5/10 (2006.01)

Patent

CA 2717083

We describe herein methods for applying physical stimuli to cells, including mesenchymal stem cells, in culture or in vivo. These methods can be applied in, and are expected to benefit subjects in, a great variety of circumstances that arise in the context of, for example, traumatic injury (including that induced by surgical procedures), wound healing (of the skin and other tissues), cancer therapies (e.g., chemotherapy or radiation therapy), tissue transplantation (e.g., bone marrow transplantation), and aging. More generally, the present methods apply where patients would benefit from an increase in the number of cells (e.g., stem cells) within a given tissue and, ex vivo, where it is desirable to increase the proliferation of cells (e.g., stem cells) for scientific study, inclusion in devices bearing cells (e.g., polymer or hydrogel-based implants), and administration to patients.

Linvention concerne des procédés dapplication de stimuli physiques à des cellules, notamment des cellules souches mésenchymateuses, en culture ou in vivo. Ces procédés peuvent être appliqués, et sont prévus pour être avantageux pour des sujets, dans diverses circonstances qui apparaissent dans le contexte par exemple dune blessure traumatique (y compris celles induites par des procédures chirurgicales), de la guérison dune blessure (de la peau et dautres tissus), de thérapies du cancer (p. ex. chimiothérapie ou radiothérapie), de la transplantation de tissus (p. ex. transplantation de moelle osseuse) et du vieillissement. Plus généralement, les présents procédés sappliquent dans les cas où des patients bénéficieraient dune augmentation du nombre de cellules (p. ex. cellules souches) dans un tissu donné et, ex vivo, dans les cas où il est souhaitable daugmenter la prolifération de cellules (p. ex. cellules souches) pour une étude scientifique, pour une inclusion dans des dispositifs comportant des cellules (p. ex. implants polymères ou à base dhydrogel) et pour une administration à des patients.

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