C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/06 (2006.01) C12Q 1/04 (2006.01) G01N 33/483 (2006.01)
Patent
CA 2236687
The present invention provides methods for rapidly determining the proportion of live, dead and stressed cells in a given microbial culture. The measurement of stressed cells is used as a quantitative indicator of the relative health of the population and the population's ability to withstand long term stress. The present methods comprise the steps of: (i) staining the culture with a combination of membrane-permeable and membrane-impermeable stains; and (ii) quantifiying the population of stressed cells, using the measurement thus obtained as an indicator of the relative health of the population.
La présente invention concerne des procédés de détermination rapide des proportions de cellules vivantes, mortes et stressées, dans une culture microbienne donnée. La mesure des cellules stressées est utilisée comme indicateur quantitatif de la santé relative de la population et de sa capacité à résister au stress prolongé. Les procédés selon l'invention comprennent : (a) la coloration de la culture avec une combinaison de colorants auxquels la membrane est perméable ou non; (b) détermination quantitative de la population de cellules stressées, la mesure ainsi obtenue étant utilisée comme indicateur de la santé relative de la population.
Harman Elizabeth K.
Rutherford William M.
Pioneer Hi-Bred International Inc.
Torys Llp
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