Methods of biosensing using fluorescent polymers and...

G - Physics – 01 – N

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Details

G01N 33/58 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/533 (2006.01) G01N 33/542 (2006.01) G01N 33/543 (2006.01) G01N 33/566 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2505907

Complexes of a biotinylated fluorescent polymer and a biotin binding protein and solid supports coated with the fluorescent polymer complexes are described. The complexes can be used as sensors for detecting biological recognition events (e.g., nucleic acid hybridization reactions or enzymatic induced polypeptide cleavage). Methods of making the complexes and methods of using the complexes for detecting the presence and/or amount of a target analyte in a sample are also described. The target analyte can be an enzyme (e.g., .beta.-secretase) or a nucleic acid (e.g., a single stranded or double stranded nucleic acid).

L'invention concerne des complexes constitués d'un polymère fluorescent biotinylé et d'une protéine se liant à la biotine, ainsi que des supports solides revêtus desdits complexes. Ces complexes peuvent être utilisés en tant que capteurs pour détecter des événements de reconnaissance biologique (par ex. des réactions d'hybridation d'acides nucléiques ou un clivage polypeptidique induit par voie enzymatique). L'invention se rapporte également à des procédés de production desdits complexes ainsi qu'à des procédés d'utilisation de ces complexes pour détecter la présence et/ou déterminer la quantité d'un analyte cible dans un échantillon. Cet analyte cible peut se présenter sous la forme d'une enzyme (par ex. une .beta.-secrétase) ou d'un acide nucléique (par ex. un acide nucléique monocaténaire ou bicaténaire).

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