C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/55 (2006.01) C12N 9/22 (2006.01) C12N 15/31 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01)
Patent
CA 2511442
Methods for specific RNA capture, detection and quantification are presented utilizing a protein that selectively binds RNA:DNA hybrids, preferably an RNase H that is modified to reduce degradation of the nucleic acid molecules and enhance specific detection of mixed RNA:DNA nucleic acid hybrids. Labeling of the RNA and/or amplification is not required to perform these methods. Modified RNase H enzymes useful in such methods are disclosed.
L'invention concerne des méthodes spécifiques de capture, de détection et de quantification d'ARN à l'aide d'une protéine qui se lie sélectivement à des hybrides d'ARN/ADN, de préférence une RNase H qui est modifiée afin de réduire la dégradation des molécules d'acide nucléique et de favoriser la détection spécifique d'hybrides d'acides nucléiques d'ARN/ADN mixtes. Le marquage de l'ARN et/ou l'amplification n'est pas nécessaire à la mise en oeuvre desdites méthodes. L'invention concerne également des enzymes de RNase H utiles dans lesdites méthodes.
Carter Jr. Richard H.
Gentry Daniel R.
Grinter Nigel
Rosenburg Martin
Promega Corporation
Smart & Biggar
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