Methods of conferring ppo-inhibiting herbicide resistance to...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 1/00 (2006.01) A01N 43/00 (2006.01) A01N 43/54 (2006.01) A01P 13/00 (2006.01) C12N 5/04 (2006.01) C12N 5/10 (2006.01) C12N 9/02 (2006.01) C12N 15/53 (2006.01) C12Q 1/02 (2006.01) C12Q 1/26 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2276053

The present invention provides methods to confer resistance to protoporphyrinogen-inhibiting herbicides onto crop plants. Resistance is conferred by genetically engineering the plants to express cloned DNA encoding a protoporphyrinogen oxidase resistant to porphyric herbicides. If such resistant crop plants are cultivated, utilization of these herbicides on fields of these crop plants becomes feasible. This should allow for simpler and more effective weed management, and increase the value of these herbicides for agricultural use. Furthermore, the present invention provides plants, algae, plant cells, and algal cells which have been made resistant to protoporphyrinogen oxidase-inhibiting herbicides by the subject methods using a herbicide- resistant protoporphyrinogen oxidase gene that has been prepared by genetic engineering methods. In addition, the present invention provides methods to evaluate the inhibitory effects of test compounds on protoporphyrinogen oxidase activity, as well as methods to identify protoporphyrinogen oxidase inhibitors among test compounds. Preferred cloned DNA fragments encoding protoporphyrinogen oxidase enzymes resistant to porphyric herbicides are also described.

La présente invention porte sur des procédés conférant aux plantes de grande culture une résistance aux herbicides inhibant un protoporphyrinogène. On confère aux plantes cette résistance en les manipulant génétiquement de façon à exprimer l'ADN cloné codant une oxydase protoporphyrinogène résistant aux herbicides porphyriques. Si l'on produit ce type de plantes de grande culture résistant aux herbicides, il est alors possible d'utiliser ces herbicides sur les champs. Cela permettrait d'éliminer plus simplement et avec plus d'efficacité les mauvaises herbes et d'accroître l'effet de ces herbicides destinés à être utilisés en agriculture. Cette invention porte en outre sur des plantes, des algues, des cellules végétales et des cellules algaires que l'on a rendu résistantes aux herbicides inhibant l'oxydase protoporphyrinogène à l'aide des procédés précités en utilisant un gène de cette oxydase protoporphyrinogène obtenu par génie génétique. L'invention porte également sur des procédés destinés à évaluer les effets inhibiteurs de composés tests sur l'activité de l'oxydase protoporphyrinogène, ainsi que sur des procédés d'identification d'inhibiteurs de l'oxydase protoporphyrinogène parmi les composés tests. L'invention porte encore sur des fragments d'ADN cloné codant les enzymes oxydases protoporphyrinogènes résistant aux herbicides porphyriques.

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