Methods of controlling gene expression and gene silencing

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 9/22 (2006.01) C07K 14/415 (2006.01) C12N 5/10 (2006.01) C12N 15/63 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2416710

The present invention relates to methods to regulate gene expression in plants. In particular, manipulation of the expression in a plant cell of a nucleotide sequence encoding a polypeptide comprising a 3'-5' exonuclease domain is disclosed. More stable and predictable expression is thus obtained. The present invention also relates to method of increasing or decreasing port- transcriptional silencing. The invention further relates to novel nucleic acid molecules comprising nucleotide sequences encoding polypeptides comprising a 3'-5' exonuclease domain.

L'invention concerne des méthodes permettant de réguler l'expression génique des plantes. L'invention concerne en particulier une manipulation de l'expression d'une cellule de séquence nucléotidique de plante codant pour un polypeptide qui comprend un domaine 3'-5' exonucléase. On obtient ainsi une expression plus stable plus prévisible. L'invention concerne également une méthode permettant d'augmenter ou de réduire une inhibition post-transcriptionnelle. L'invention concerne enfin de nouvelles molécules d'acide nucléique comprenant des séquences nucléotidiques qui codent pour des polypeptides renfermant un domaine 3'-5' exonucléase

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