Methods of detecting interactions between proteins, peptides...

Details Methods of detecting interactions between proteins, peptides... Methods of detecting interactions between proteins, peptides...

G - Physics

01

N

G01N 33/68 (2006.01) C07K 14/435 (2006.01) C07K 14/47 (2006.01) C07K 19/00 (2006.01) C12N 15/12 (2006.01)

Patent

CA 2384561

The present invention discloses a method of reconstituting, folding, or reassembling peptides or other binding pairs into a functionally active protein or other complex using an antiparallel leucine zipper. The present invention also provides assays using fusion proteins comprising GFP fragments and test polypeptides for investigating protein-protein interactions.

L'invention concerne une méthode permettant de reconstituer, de plier ou de réassembler des peptides ou d'autres paires de liaison en une protéine fonctionnellement active ou en un autre complexe au moyen d'une glissière à leucine antiparallèle. L'invention concerne également des dosages utilisant de protéines de fusion contenant des fragments GFP et des polypeptides d'essai permettant de détecter les interactions protéine-protéine.

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