Methods of detecting modification of genetic material

Details Methods of detecting modification of genetic material Methods of detecting modification of genetic material

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) A61K 45/00 (2006.01) A61P 31/04 (2006.01) A61P 31/12 (2006.01) A61P 43/00 (2006.01) C12N 15/09 (2006.01) C12Q 1/25 (2006.01) C12Q 1/42 (2006.01) C12Q 1/48 (2006.01) C12Q 1/533 (2006.01) G01N 21/77 (2006.01) G01N 21/78 (2006.01) G01N 33/15 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01) G01N 33/566 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2470315

The invention relates to a method for determining the activity of an enzyme or enediyne capable of altering the structure of a "substrate" nucleic acid from a first to a second state wherein the activity of the enzyme or enedyine is monitored using a chemiluminescent label that is either attached to the "substrate" nucleic acid or an oligonucleotide which is complementary thereto or the enzyme or enediyne product thereof.

L'invention se rapporte à un procédé permettant de déterminer l'activité d'une enzyme ou d'une enediyne pouvant faire passer la structure d'un acide nucléique <= substrat >= d'un premier état à unsecond état, l'activité de l'enzyme ou de l'enediyne étant contrôlée au moyen d'un traceur chimioluminescent, fixé soit à l'acide nucléique <= substrat >=, soit à un oligonucléotide qui lui est complémentaire, soit au produit d'enzyme ou d'enediyne dudit acide nucléique.

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