Methods of detecting nucleic acids in individual cells and...

Details Methods of detecting nucleic acids in individual cells and... Methods of detecting nucleic acids in individual cells and...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2612577

Methods of detecting multiple nucleic acid targets in single cells through indirect capture of labels to the nucleic acids are provided. Methods of assaying the relative levels of nucleic acid targets through normalization to levels of reference nucleic acids are also provided. Methods of detecting individual cells, particularly rare cells from large heterogeneous cell populations, through detection of nucleic acids are described. Related compositions, systems, and kits are also provided.

L'invention porte sur des méthodes de détection de plusieurs cibles d'acides nucléiques dans des cellules uniques par capture indirecte d'étiquettes sur les acides nucléiques. L'invention porte également sur des méthodes de dosage des taux relatifs des cibles d'acides nucléiques par normalisation par rapport aux taux des acides nucléiques de référence. L'invention porte sur des méthodes de détection de cellules individuelles, en particulier de cellules rares à partir de grandes populations cellulaires hétérogènes par détection des acides nucléiques. L'invention porte encore sur des compositions, sur des systèmes et sur des kits correspondants.

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