Methods of detecting polynucleotide analytes

Details Methods of detecting polynucleotide analytes Methods of detecting polynucleotide analytes

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2312192

Sensitive and specific methods of detecting single-stranded polynucleotide analytes can be used in manual or automated diagnostic assays and to screen blood samples for the presence of infectious agents. The methods can be used to detect any single-stranded polynucleotide analyte whose sequence is known. Single-stranded DNA analytes which are present in a biological sample at a concentration of 0.1 fg/µl can be detected.

L'invention concerne des procédés sensibles et spécifiques de détection d'analytes polynucléotidiques monocaténaires qui peuvent être utilisés pour des analyses diagnostiques manuelles ou automatisées et pour le dépistage d'agents infectieux dans des échantillons sanguins. Ces procédés peuvent être utilisés pour détecter tout analyte polynucléotidique monocaténaire dont la séquence est connue. Les analytes d'ADN monocaténaire présents dans un échantillon biologique à une concentration de 0,1 fg/µl peuvent être détectés.

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