Methods of detection of amyloidogenic proteins

Details Methods of detection of amyloidogenic proteins Methods of detection of amyloidogenic proteins

G - Physics

01

N

G01N 33/68 (2006.01)

Patent

CA 2395789

A method/assay for detecting various aggregated forms of amyloid peptide using amyloid-specific spectroscopic probes, such as Congo red and related analogs, is described. Such an assay could be used to assess the aggregation state of the peptide in the presence of other potential inhibitors of fibrillogenesis, providing a useful model by which to test inhibitors of aggregation in in vitro models. Results of such an assay can be useful in determining the stage at which the process of aggregation can be intervened to reduce the toxicity of amyloid peptide to neuronal cells. In specific examples, circular dichroism, UV absorption, and fluorescence spectroscopy are each used to evaluate the aggregation form of .beta.-amyloid peptide.

Procédé/dosage destiné à la détection de diverses formes agrégées du peptide amyloïde en utilisant des sondes spectroscopiques spécifiques aux amyloïdes telles que le rouge de Congo et les analogues correspondants. Ce dosage peut s'utiliser pour faire une estimation de l'état d'agrégation du peptide en présence d'autres inhibiteurs potentiels de la fibrillogenèse; il constitue un modèle utile permettant de tester les inhibiteurs d'agrégation dans des modèles in vitro. Les résultats de ce dosage peuvent être utiles pour déterminer le stade auquel on peut intervenir dans le processus d'agrégation pour réduire la toxicité du peptide amyloïde vis-à-vis des cellules neurales. Dans des exemples concrets, on utilise le dichroïsme circulaire, l'absorption des U.V. et la spectroscopie par fluorescence pour évaluer la forme d'agrégation du peptide beta -amyloïde.

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