Methods of determining properties of molecules

Details Methods of determining properties of molecules Methods of determining properties of molecules

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) G06F 19/20 (2011.01)

Patent

CA 2723980

This disclosure features, inter alia, methods for determining at least one property of a nucleic acid. The methods include: (a) fixing the nucleic acid on a planar surface; (b) digesting the nucleic acid into fragments with at least one enzyme; (c) imaging the nucleic acid; and (d) analyzing the imaged nucleic acid to determine the property of the nucleic acid, wherein no internal nucleic acid standard is added during the method.

Linvention concerne entre autres des procédés pour déterminer au moins une propriété dun acide nucléique. Les procédés comprennent : (a) la fixation de lacide nucléique sur une surface plane; (b) la digestion de lacide nucléique en fragments avec au moins une enzyme; (c) limagerie de lacide nucléique; et (d) lanalyse de lacide nucléique imagé pour déterminer la propriété de lacide nucléique, aucun standard dacide nucléique interne étant ajouté pendant le procédé.

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