C - Chemistry – Metallurgy – 07 – H
Patent
C - Chemistry, Metallurgy
07
H
C07H 21/00 (2006.01) C07H 21/04 (2006.01) C12N 15/10 (2006.01)
Patent
CA 2375215
The present invention provides methods for the purification of plasmid DNA that includes removal of host cell impurities, such as endotoxins, RNA, proteins, and chromosomal DNA, from an aqueous solution containing plasmid DNA and methods for separation and purification of supercoiled plasmid DNA from an aqueous solution containing a mixture of supercoiled and nicked or relaxed plasmid DNA using hydrophobic interaction chromatography supports.
La présente invention concerne des procédés de purification d'ADN plasmidique consistant à éliminer les impuretés de cellules hôtes, telles que endotoxines, ARN, protéines, et ADN chromosomique, d'une solution aqueuse contenant de l'ADN plasmidique, et concerne en outre des procédés de séparation et de purification d'ADN plasmidique superenroulé provenant d'une solution aqueuse contenant un mélange d'ADN plasmidique superenroulé et entaillé ou déroulé, au moyen de supports de chromatographie hydrophobe.
Bio Science Contract Production Corporation
Goudreau Gage Dubuc
Lonza Biologics Inc.
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