Methods of expressing lim mineralization protein in...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/85 (2006.01) A61F 2/44 (2006.01) A61K 31/7088 (2006.01) A61K 48/00 (2006.01) A61L 27/38 (2006.01) A61P 19/00 (2006.01) C07K 14/47 (2006.01) C07K 14/78 (2006.01) C07K 16/18 (2006.01) C12N 5/10 (2006.01) C12N 15/86 (2006.01) A61K 35/12 (2006.01)

Patent

CA 2466769

Methods of expressing LIM mineralization protein in non-osseous mammalian cells, such as stem cells or intervertebral disc cells (e.g., cells of the annulus fibrosus, or cells of the nucleus pulposus) are described. The methods involve transfecting the cells with an isolated nucleic acid comprising a nucleotide sequence encoding a LIM mineralization protein operably linked to a promoter. Transfection may be accomplished ex vivo or in vivo by direct injection of virus or naked DNA, or by a nonviral vector such as a plasmid. Expression of the LIM mineralization protein can stimulate proteoglycan and/or collagen production in cells capable of producing proteoglycyan and/or collagen. Methods for treating disc disease associated with trauma or disc degeneration are also described.

L'invention concerne des procédés d'expression de protéine de minéralisation LIM dans des cellules mammifères non osseuses, telles que des cellules souches ou des cellules de disque intervertébral (par exemple, cellules de l'anneau fibreux du disque intervertébral, ou cellules du noyau gélatineux). Ces procédés comprennent la transfection des cellules avec un acide nucléique isolé présentant une séquence nucléotide codant pour une protéine de minéralisation LIM liée coopérante à un promoteur. La transfection peut être effectuée ex vivo ou in vivo par injection directe d'un virus ou d'ADN nu, ou par un vecteur non viral tel qu'un plasmide. L'expression de la protéine de minéralisation LIM peut stimuler la production de protéoglycane et/ou de collagène dans des cellules capables de produire du protéoglycane et/ou du collagène. L'invention concerne également des procédés de traitement de discopathie associée à des traumatismes ou à des dégénérations discales.

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