C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
150/8.5
C12Q 1/68 (2006.01) C07K 14/16 (2006.01) C07K 14/74 (2006.01) C07K 14/805 (2006.01) C12N 15/10 (2006.01)
Patent
CA 2013316
-0- METHODS OF EXTRACTING, AMPLIFYING AND DETECTING A NUCLEIC ACID FROM WHOLE BLOOD OR PBMC FRACTION Abstract of the Disclosure A nucleic acid is rapidly extracted from whole blood or a peripheral blood mononuclear cell (PBMC) fraction thereof. Extraction from the PBMC fraction is accomplished by heating the fraction at or near the boiling point of water for a few minutes and recovering the extracted nucleic acid. This rapid method is particularly useful for extracting DNA for the detection of genetic diseases or infectious agents, such as HIV-I. Whole blood can likewise be heated after it is mixed with a salt solution containing a polysaccharide, such as dextran. The extracted nucleic acid is then recovered from the heated mixture. Nucleic acids extracted in this way are available for amplification using a polymerase chain reaction. Where the presence of a specific gene is to be determined for diagnostic purposes, it can be extracted as described above and subjected to suitable amplification and detection steps.
Burdick Brent A.
Ekeze Tobias D.
Burdick Brent A.
Eastman Kodak Company
Ekeze Tobias D.
Gowling Lafleur Henderson Llp
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