Methods of gene amplification and expression

Details Methods of gene amplification and expression Methods of gene amplification and expression

C - Chemistry, Metallurgy

12

P

C12P 21/02 (2006.01) C12N 5/10 (2006.01) C12N 15/09 (2006.01) C12N 15/67 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2682482

Disclosed are methods relating to amplification and expression of a nucleic acid sequence encoding a polypeptide of interest in recombinant cells, and cell lines and polypeptides produced from such methods. The methods disclosed herein permit the amplification of cell lines that express a polypeptide of interest in a relatively short period of time through the use of a bioreactor.

Procédés d'amplification et d'expression de séquence nucléotidique codant un polypeptide spécifique dans des cellules de recombinaison, et lignées cellulaires et polypeptides résultants. Les procédés décrits permettent l'amplification de lignées cellulaires qui expriment un polypeptide spécifique dans un laps de temps relativement bref, par le biais d'un bioréacteur.

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