C - Chemistry – Metallurgy – 40 – B
Patent
C - Chemistry, Metallurgy
40
B
C40B 40/00 (2006.01) A61K 9/127 (2006.01) A61K 47/48 (2006.01) C12Q 1/02 (2006.01) C40B 30/04 (2006.01) C40B 40/02 (2006.01) C40B 40/10 (2006.01) G01N 33/567 (2006.01) G01N 33/58 (2006.01) G01N 33/68 (2006.01) C07K 7/06 (2006.01) C40B 70/00 (2006.01)
Patent
CA 2425602
This invention provides methods of identifying ligands that are internalized into a cell. The methods typically involve (i) contacting the cell with a reporter non-covalently coupled to a ligand; (ii) dissociating the reporter from the ligand and removing dissociated reporter from the surface of the cell; and (iii) detecting the reporter within said cell (if any is present) where the presence of the reporter within said cell indicates that the ligand binds to an internalizing receptor and is internalized.
La présente invention concerne des procédés servant à identifier des ligands qui sont internalisés dans une cellule. Lesdits procédés comprennent en général: (i) la mise en contact de la cellule avec un reporteur couplé par liaison non covalente à un ligand; (ii) la dissociation entre le reporteur et le ligand et l'élimination du reporteur dissocié de la surface de la cellule; et (iii) la détection du reporteur à l'intérieur de ladite cellule (si présent), la présence du reporteur à l'intérieur de ladite cellule indiquant que le ligand se lie à un récepteur d'internalisation et est internalisé.
Kirpotin Dmitri B.
Marks James D.
Nielsen Ulrik B.
Smart & Biggar
The Regents Of The University Of California
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