Methods of identification and isolation of polynucleotides...

Details Methods of identification and isolation of polynucleotides... Methods of identification and isolation of polynucleotides...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2425545

The present invention is directed to methods of isolation of related polynucleotides harboring nucleic acid difference within a polynucleotide sample. The method will be useful in detecting and identifying alternative splicing events and corresponding splicing isoforms and to detect genomic DNA differences between genomes. The method according to the present invention is based on the use of a single-stranded trap. The single-stranded trap preferably involves the use of single-strand binding protein.

La présente invention concerne des procédés permettant d'isoler des polynucléotides apparentés présentant des différences d'acide nucléique à l'intérieur d'un échantillon polynucléotidique. Ce type de procédé peut être utile pour détecter et identifier des événements d'épissage alternatif et des isoformes d'épissage correspondantes et pour détecter des différences d'ADN génomique entre des génomes. Le procédé selon la présente invention repose sur l'utilisation d'un piège à brin unique, faisant appel de préférence à une protéine de liaison à brin unique.

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