Methods of identifying compounds that modulate a dna repair...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/867 (2006.01) A61K 45/00 (2006.01) A61P 31/12 (2006.01) C07K 14/47 (2006.01) C12Q 1/02 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2480437

Retroviruses are RNA viruses that must insert a DNA copy (cDNA) of their genome into the host genome in order to carryout a productive infection. One host cellular pathway that defends against retroviral cDNA integration involves highly conserved proteins of a host DNA repair pathway. These proteins represent novel targets for anti-retroviral drugs. The invention presented herein provides, inter alia, methods of identifying compounds that induce a DNA repair pathway and/or inhibit retroviral cDNA integration into a host genome, compounds thus identified, uses of such compounds, and kits for identifying and testing of the efficacy of compounds in inducing a DNA repair pathway, inhibiting retroviral cDNA integration, and inhibiting retroviral infection.

Des rétrovirus sont des virus à ARN qui doivent introduire une copie d'ADN (ADNc) dans leur génome et dans le génome hôte de façon à conduire une infection productive. Une voie cellulaire hôte qui se défend contre l'intégration de l'ADNc rétroviral fait intervenir des protéines hautement conservées d'une voie de réparation d'ADN hôte. Ces protéines représentent de nouvelles cibles pour des médicaments anti-rétroviraux. La présente invention concerne, entre autres choses, des techniques d'identification de composés qui induisent une voie de réparation d'ADN et/ou inhibent l'intégration de l'ADNc rétroviral dans un génome hôte, des composés ainsi identifiés, des utilisations de ces composés et des kits permettant d'identifier et de tester l'efficacité de ces composés pour ce qui concerne l'induction d'une voie de réparation d'ADN, l'inhibition de l'intégration de l'ADNc rétroviral et l'inhibition de l'infection rétrovirale.

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