Methods of identifying immunoregulatory agents,...

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/113 (2010.01) A61K 31/713 (2006.01) C12N 15/11 (2006.01) C12Q 1/00 (2006.01) G01N 33/15 (2006.01) G01N 33/53 (2006.01) G01N 33/68 (2006.01) C07H 21/02 (2006.01)

Patent

CA 2551912

The present invention provides methods of identifying a substance that down- regulates or up-regulates an immune response and kits used for the identification methods. A method of this invention comprises detecting a substance that inhibits or enhances the interaction between CD26 and caveolin- 1. Another method comprises detecting a substance that inhibits or enhances the interaction between caveolin-1 and Tollip. Still another method comprises detecting a substance that inhibits or enhances the interaction among caveolin- 1, Tollip, and IRAK-1. The present invention also relates to immunoregulatory agents comprising a substance that down-regulates an immune response, such as siRNA against caveolin-1 or Tollip. The invention further provides immunoregulatory agents comprising a substance that up-regulates an immune response. These agents are useful for treating inflammatory diseases, autoimmune diseases, or other immune-mediated disorders.

L'invention concerne des méthodes d'identification d'une substance qui régule à la hausse ou à la baisse une réaction immunitaire et des kits utilisés dans ces méthodes d'identification. Une méthode de l'invention consiste à détecter une substance qui inhibe ou qui accroît l'interaction entre CD26 et cavéoline-1. Une autre méthode consiste à détecter une substance qui inhibe ou qui accroît l'interaction entre cavéoline-1 et Tollip. Une autre méthode consiste à détecter une substance qui inhibe ou qui accroît l'interaction entre cavéoline-1, Tollip, et IRAK-1. L'invention concerne également des agents immunorégulateurs comprenant une substance qui régule à la baisse une réaction immunitaire, de type petit ARN interférent contre cavéoline-1 ou Tollip. L'invention concerne en outre des agents immunorégulateurs comprenant une substance qui régule à la hausse une réaction immunitaire. Ces agents servent à traiter des maladies inflammatoires, des maladies auto-immunes, ou d'autres troubles à médiation immunitaire.

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