C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/04 (2006.01) C12N 15/09 (2006.01) C12N 15/63 (2006.01)
Patent
CA 2724468
Disclosed herein are methods of identifying inhibitors of gene silencing or re- silencing, which can include repress-ing expression of a selectable marker gene in mammalian cells, treating the cells with at least one test compound, growing the cells under selective conditions, and quantifying the relative number of cells that live, wherein a change in the relative number of cells as compared to cells that were not treated with the test compound, identifies the compound as an inhibitor of gene silencing or re-silencing. Also disclosed herein are transgenic mice and isolated cell lines that are useful in the disclosed methods and kits for use in performing the disclosed methods.
La présente invention concerne des procédés didentification des inhibiteurs du silençage ou du re-silençage génique, pouvant comprendre la répression de lexpression dun marqueur génétique sélectionnable dans des cellules de mammifère, le traitement des cellules au moyen dau moins un composé de test, la croissance des cellules dans des conditions sélectives, et la quantification du nombre relatif de cellules vivantes, une modification dudit nombre relatif de cellules par rapport aux cellules non traitées au moyen du composé de test permettant didentifier ledit composé en tant quinhibiteur du silençage ou du re-silençage génique. Linvention porte également sur des souris transgéniques et sur des lignées cellulaires isolées se révélant utiles dans le cadre des procédés décrits, ainsi que des kits à utiliser pour lexécution desdits procédés.
Goudreau Gage Dubuc
Oregon Health & Science University
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