Methods of identifying optimal variants of peptide epitopes

G - Physics – 01 – N

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G01N 33/68 (2006.01)

Patent

CA 2520768

The present invention is directed to methods for selecting a variant of a peptide epitope which induces a CTL response against another variant(s) of the peptide epitope, by determining whether the variant comprises only conserved residues, as defined herein, at non-anchor positions in comparison to the other variant(s). The present invention is also directed to variants identified by the methods above; peptides comprising such variants; nucleic acids encoding such variants and peptides; cells comprising such variants, and/or peptides, and/or nucleic acids; compositions comprising such variants, and/or peptides, and/or nucleic acids, and/or cells; as well as therapeutic and diagnostic methods for using such variants, peptides, nucleic acids, cells, and compositions.

La présente invention a trait à des procédés permettant la sélection d'un variant d'un épitope peptidique induisant une réponse CTL contre un autre variant ou d'autres variants de l'épitope peptidique, en déterminant si le variant comporte uniquement des résidus préservés, tels que définis dans la description, à des positions de non ancrage par rapport à l'autre variant ou aux autres variants. La présente invention a également trait à des variants identifiés par lesdits procédés, des peptides comportant de tels variants ; à des acides nucléiques codant pour de tels variants et peptides ; à des cellules comprenant de tels variants, et/ou à des peptides, et/ou à des acides nucléiques ; à des compositions comportant de tels variants, et/ou à des peptides, et/ou à des acides nucléiques, et/ou à des cellules ; ainsi qu'à des procédés thérapeutiques et diagnostiques pour l'utilisation de tels variants, peptides, acides nucléiques, cellules, et compositions.

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