C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12N 15/10 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01)
Patent
CA 2382121
A method is provided for immobilizing a ligand, e.g., a nucleic acid, on a solid support. The method includes providing a solid support containing an immobilized latent thiol group, activating the thiol group, contacting the activated thiol group with a nucleic acid comprising an acrylamide functional group, and forming a covalent bond between the two groups, thereby immobilizing the nucleic acid to the solid support. Kits containing the solid supports and method of utilizing the solid supports are also provided.
La présente invention concerne un procédé permettant d'immobiliser un ligand, par exemple un acide nucléique, sur un support solide. Le procédé consiste à se munir d'un support solide contenant un groupe thiol latent immobilisé, à activer le groupe thiol, à mettre en contact le groupe thiol activé avec un acide nucléique comprenant un groupe acrylamide, et à former la liaison covalente entre les deux groupes, immobilisant ainsi l'acide nucléique sur le support solide. La présente invention concerne également des nécessaires contenant les supports solides et un procédé d'utilisation de tels supports.
Abrams Ezra S.
Mielewczyk Slawomir
Patterson Brian C.
Zhang Tianhong
Macrae & Co.
Matrix Technologies Corporation
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