Methods of immobilizing oligonucleotides to solid support...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2207629

The present invention provides a method for directly immobilizing an oligonucleotide to a support material. The method comprises the steps of contacting a solution of oligonucleotides with a solid support material and drying the oligonucleotide solution to the support material. Oligonucleotides and the solid support materials to which they are immobilized can be employed as capture reagents for immobilizing nucleic acid sequences which are complementary to the immobilized oligonucleotides. Hence, the hybridization capacity of directly immobilized oligonucleotides is maintained. Also provided are methods for determining the presence or amount of nucleic acid sequences in a test sample.

Procédé d'immobilisation directe d'un oligonucléotide sur un matériau support consistant à mettre en contact une solution d'oligonucléotides avec un matériau support solide puis à dessécher ladite solution sur le support. Les oligonucléotides et leur support d'immobilisation peuvent servir de réactifs de capture pour l'immobilisation des séquences nucléotidiques complémentaires des oligonucléotides immobilisés. On maintient de cette façon la capacité d'hybridation des oligonucléotides directement immobilisés. Sont également présentés des procédés de détermination de la présence ou du volume de séquences nucléotidiques dans un échantillon d'essai.

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