Methods of immunoassay

Details Methods of immunoassay Methods of immunoassay

G - Physics

01

N

167/45

G01N 33/53 (2006.01) G01N 33/535 (2006.01) G01N 33/58 (2006.01)

Patent

CA 1284104

ABSTRACT Methods of Immunoassay The present invention relates to enzyme immunoassays employing one or more enzyme label-substrate pairs which give rise to coloured products and in which absorption by the product of substrate conversion by at least one enzyme label at a first wavelength, at or close to the optimum wavelength for absorbance, exceeds the linear range of the detector, which immunoassay includes the steps of measuring the said substrate conversion at a second wavelength at which absorbance by the relevant product is significantly lower than at the said first wavelength and calculating the true absorbance at the said first wavelength by utilizing the result of linear regression analysis of absorbance measurements obtained with product standards at the said first and second wavelengths within the linear range of the detector.

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