Methods of in vitro propagation and detection of infectious...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/0784 (2010.01) C07K 14/47 (2006.01) C07K 14/705 (2006.01) C12P 21/00 (2006.01) G01N 33/53 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2632662

Methods for the in vitro propagation of infectious prions (PrPSc) are provided. Follicular dendritic cells (FDCs) are cultured with B cells and infected with prions. Methods of detecting infectious prions (PrPSc) in an animal or human are also provided. Peripheral blood B cells are collected from an animal or human suspected of being infected with infections prions, cultured with follicular dendritic cells, and the presence of infectious prions is detected.

L'invention concerne des procédés de propagation in vitro de prions infectieux (PrPSc). Des cellules dendritiques folliculaires (FDC) sont mises en culture avec des cellules B et infectées par des prions. Cette invention concerne également des procédés de détection de prions infectieux (PrPSc) chez un animal ou un être humain. Des cellules B du sang périphérique sont prélevées sur un animal ou un être humain présumé infecté par des prions infectieux puis mises en culture avec des cellules dendritiques folliculaires et la présence de prions infectieux est détectée.

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