Methods of in vivo gene delivery

C - Chemistry – Metallurgy – 12 – N

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C12N 15/86 (2006.01) C12N 15/863 (2006.01)

Patent

CA 2189452

We have discovered that using non-integrative viral vectors having low replicative efficiency for insertion of a gene into a cell such as a lymphocyte or a tumor cell is a preferred system for transforming such cells for use in somatic cell therapy or gene therapy. These vectors are preferably cytoplasmic viral vectors, as opposed to nuclear viral vectors. Preferred cytoplasmic vectors include DNA viruses such as pox viruses and iridoviruses and RNA viruses such as picornavirus, calicivirus and togavirus. More preferably the virus used will not be capable of sustained replication in the target cell. For example, a preferred pox virus for human cells will be an avipox, or suipox in contrast to an orthopox virus such as vaccinia.

On a découvert que l'utilisation de vecteurs viraux non intégratifs à faible efficacité de réplication pour l'insertion d'un gène dans une cellule telle qu'une cellule lymphocytaire ou tumorale constitue un système préféré pour la transformation de ces cellules en vue de leur utilisation dans la thérapie cellulaire somatique ou la thérapie génique. Ces vecteurs sont, de préférence, des vecteurs viraux cytoplasmiques, par opposition à des vecteurs viraux nucléaires. Les vecteurs cytoplasmiques préférés englobent les virus d'ADN tels que les virus pox et les iridovirus, et les virus d'ARN tels que le picornavirus, le calicivirus et le togavirus. Idéalement, le virus utilisé n'est pas être capable de soutenir la réplication dans la cellule cible. Par exemple, un virus pox préféré pour les cellules humaines sera un avipox ou un suipox et non pas un virus orthopox tel que la vaccine.

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