Methods of increasing rates of infection by directing motion...

Details Methods of increasing rates of infection by directing motion... Methods of increasing rates of infection by directing motion...

C - Chemistry, Metallurgy

12

N

C12N 15/64 (2006.01) C12M 1/00 (2006.01) C12M 3/00 (2006.01) C12M 3/06 (2006.01) C12N 15/87 (2006.01)

Patent

CA 2172992

This invention provides methods of increasing the frequency of contact between vectors and stationary target cells in an apparatus containing them which involves causing the vectors to move towards the target cells with motion above and beyond random Brownian motion. The methods of this invention include causing the vectors to move in the direction of the cells by (1) causing flow-through of a liquid containing the vectors through or past a cell bed, (2) moving charged vectors towards the target cells by electrodiffusion and (3) centrifuging vectors and cells to cause settling of vectors onto the cells.

Le procédé de la présente invention permet d'accroître la fréquence de contact entre les vecteurs et des cellules cibles stationnaires placées à l'intérieur d'un appareil. Cet appareil a pour effet de provoquer un déplacement des vecteurs vers les cellules cibles grâce à un mouvement supérieur et au-delà du mouvement aléatoire Brownien. Le procédé de la présente invention permet également de provoquer le déplacement des vecteurs en direction des cellules, 1~) en provoquant la circulation d'un liquide contenant les vecteurs au travers ou au-delà d'un lit de cellules, 2~) en déplaçant par électrodiffusion des vecteurs chargés en direction des cellules cibles, et 3~) en centrifugeant les vecteurs et les cellules pour provoquer le dépôt des vecteurs sur les cellules.

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