Methods of inducing differentiation of stem cells into a...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/02 (2006.01) A61K 35/14 (2006.01) A61K 35/23 (2006.01) A61K 35/28 (2006.01) A61K 35/30 (2006.01) A61K 35/34 (2006.01) A61K 35/36 (2006.01) A61K 35/39 (2006.01) A61K 35/42 (2006.01) A61K 35/44 (2006.01) A61K 35/48 (2006.01) A61K 48/00 (2006.01) C12N 5/06 (2006.01) C12N 5/08 (2006.01) C12N 5/10 (2006.01) A61K 35/12 (2006.01)

Patent

CA 2479152

The present invention relates to methods of inducing differentiation of stem cells into a specific cell lineage, preferably lung cells. In particular, the invention relates to in vitro methods of inducing differentiation of stem cells into a specific cell lineage. The invention also relates to methods of producing and recovering differentiated stem cells of a specific cell lineage. The invention also includes differentiated stem cells and cell lineages produced by the methods of the present invention.In one aspect of the present invention there is provided a method of inducing differentiation of a stem cell into a specific cell lineage, preferably lung cells, the method including:culturing a stem cell in vitro in the presence of a tissue sample and/or extracellular medium of a tissue sample, under conditions that induce differentiation of the stem cell into a specific cell lineage, wherein the differentiated stem cell is the same cell type as the tissue sample.

L'invention concerne des méthodes d'induction de la différenciation de cellules souches dans une lignée de cellules spécifiques, de préférence, des cellules pulmonaires. En particulier, l'invention concerne des méthodes in vitro de différenciation de cellules souches dans une lignée de cellules spécifiques. Par ailleurs, l'invention concerne des méthodes de production et de récupération de cellules souches différenciées d'une lignée de cellules spécifiques. Elle concerne en outre des cellules souches différenciées et des lignées cellulaires obtenues par les méthodes de l'invention. Dans un premier aspect, l'invention concerne une méthode d'induction de la différenciation d'une cellule souche dans une lignée de cellules spécifiques, de préférence, des cellules pulmonaires. Cette méthode consiste à mettre en culture une cellule souche in vitro en présence d'un échantillon de tissu et/ou d'un milieu extracellulaire d'un échantillon de tissu, dans des conditions qui induisent la différentiation de la cellule souche dans la lignée de cellules spécifiques. La cellule souche différenciée appartenant au même type de cellule que l'échantillon de tissu.

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